Who wants happiness,
when you can have something to strive for?
Ignatius Farray
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Hello!
I am Sebastián Sosa-Carrillo, welcome to my personal web page, here you can browse through the different sections that I have prepared for you to know a little more about me. Specifically, you will find a section where I describe a bit about my life path until today, and another one where I talk about my career as a scientific researcher. The goal is that you get to know my interests and, why not, we can contact each other if we have something to contribute. In this page, I also share links to content about news, posts, or articles that I find or that I generate and I find interesting.
Finally, if you want to contact me or give me your opinion, I appreciate it. You can do so via email, LinkedIn or Twitter. Click on any of the icons below this text to get to the link.
Thank you very much for your attention!
Highlights:
Highlights:
In this first post I am going to share the procedure for cloning and the detailed protocol for using modular cloning (MoClo) based on Golden Gate assembly. This approach is very convenient when you want to insert and shuffle several transcriptional units within the same backbone.
Competent cells and transformation:
In this post I share the procedure for making competent E. coli cells and transforming them to take up plasmids, ideally generated following the procedure explained in the first post of this series. Thus, this approach is convenient when you have several constructs to transform, they are not too complex, and you are dealing with a host that is not a weirdo.
Yeast (S. cerevisiae) transformation:
In this post I share a simple and efficient procedure to incorporate exogenous DNA into Saccharomyces cerevisiae cells, whether the goal is to integrate DNA into the chromosome or to introduce an episomal plasmid. In the context of this series of posts, this procedure would come after obtaining the DNA intended to be transformed into yeast from a miniprep of the E. coli cells generated in the second post.
Yeast (S. cerevisiae) colony PCR:
In this last post on the process of engineering a yeast strain, I share a simple procedure to perform PCR directly from yeast colonies on an agar plate, namely yeast colony PCR. It is a very common procedure performed on bacterial colonies, but with yeast it is a bit tricky. Colony PCR is intended to detect positive clones beyond phenotype, and is the step one would do after transforming the cells, as shown in the third post in this series.
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